PCR Applications,: Protocols for Functional Genomics

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9780123721860: PCR Applications,: Protocols for Functional Genomics

PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily. "PCR Applications" examines the latest developments in this field. It is the third book in the series, building on the previous publications "PCR Protocols" and "PCR Strategies". The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. It covers quantitative PCR techniques, including the use of standards and kinetic analysis includes statistical refinement of primer design parameters; and, illustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR. The entries provide information on: nomenclature, expression, sequence analysis, structure and function, electrophysiology, parmacology, and information retrieval.

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Praise for PCR Strategies: "This book succeeds in its goals of providing advanced concepts and procedures for PCR to researchers in both the basic and clinical sciences. This book contains the efforts of a number of laboratories from both industry and academics that are prominent in the field of PCR applications, and it provides a useful companion to the earlier book." Received 4 Star Rating --DOODY'S PUBLISHING REVIEWS Praise for PCR Protocols "For an up-to-date, definitive discussion of technical variations and the wide range of known applications, PCR Protocols is an invaluable reference... While PCR is in its infancy, this work will certainly be updated many times and by many others, however as a reflection of the scope of PCR to date, it will not be replaced or rendered obsolete." --APPLIED CYTOGENETICS "PCR protocols is an excellent lab manual for making PCR work... The breadth of the applications and methodology described in this book should give even the novice the confidence and skill to make PCR work... In summary, if you have questions about PCR, PCR Protocols probably has the answers." --TRENDS IN GENETICS "This volume owes its value to the expertise of its contributors, all specialists of international renown in the developing field of PCR... This book is an excellent compilation for all who, having a general background in molecular biology, wish to practice PCR...No user of the PCR technique could wish to miss it." --TIBTECH

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