Pcr Applications: Protocols for Functional Genomics - Hardcover

Hardcover

ISBN 10: 0123721857 ISBN 13: 9780123721853

Publisher: Academic Pr, 1999

Synopsis

Scientists from universities, industry, and government agencies, mostly in the US but also in Europe explain the methods for using the polyermase chain reaction technique in mRNA quantitation, diagnosis, gene discovery, genomic analysis, and expression profiling. They set out the key concepts such as thermostable DNA polymerases and optimization guidelines, and general principles of practice, then focus on specific applications. Among them are whole cell assays, comparative genomic hybridization, and the cleavase I enzyme for mutation and polymorphism scanning. The collection of articles augments rather than replaces the earlier PCR Protocols and PCR Strategies by updating theoretical notions and research experience but not duplicating the actual protocols described. Annotation c. Book News, Inc., Portland, OR (booknews.com)

About this title

Review

Praise for PCR Strategies:
"This book succeeds in its goals of providing advanced concepts and procedures for PCR to researchers in both the basic and clinical sciences. This book contains the efforts of a number of laboratories from both industry and academics that are prominent in the field of PCR applications, and it provides a useful companion to the earlier book."
Received 4 Star Rating
--DOODY'S PUBLISHING REVIEWS
Praise for PCR Protocols
"For an up-to-date, definitive discussion of technical variations and the wide range of known applications, PCR Protocols is an invaluable reference... While PCR is in its infancy, this work will certainly be updated many times and by many others, however as a reflection of the scope of PCR to date, it will not be replaced or rendered obsolete."
--APPLIED CYTOGENETICS
"PCR protocols is an excellent lab manual for making PCR work... The breadth of the applications and methodology described in this book should give even the novice the confidence and skill to make PCR work... In summary, if you have questions about PCR, PCR Protocols probably has the answers."
--TRENDS IN GENETICS
"This volume owes its value to the expertise of its contributors, all specialists of international renown in the developing field of PCR... This book is an excellent compilation for all who, having a general background in molecular biology, wish to practice PCR...No user of the PCR technique could wish to miss it."
--TIBTECH

From the Back Cover

The polymerase chain reaction is the most powerful technique currently used in molecular biology laboratories. From its discovery in the early 80s, PCR has blossomed into a method that enables everything from ready mutation of DNA to speedy analysis of tens of thousands of nucleotide sequences daily.
PCR Applications: Protocols for Functional Genomics examines the latest PCR implementations. It complements the other books in the series, PCR Protocols (1990) and PCR Strategies (1995), and techniques discussed are relevant to the now-occurring bioinformatics and proteomics boom.
Key Features:
* focuses on gene discovery, genomics, and DNA array technology
* covers quantitative PCR techniques, including the use of standards and kinetic analysis
* includes statistical refinement of primer design parameters
* illustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR|The polymerase chain reaction is the most powerful technique currently used in molecular biology laboratories. From its discovery in the early 80s, PCR has blossomed into a method that enables everything from ready mutation of DNA to speedy analysis of tens of thousands of nucleotide sequences daily.
PCR Applications: Protocols for Functional Genomics examines the latest PCR implementations. It complements the other books in the series,PCR Protocols (1990) and PCR Strategies (1995), and techniques discussed are relevant to the now-occurring bioinformatics and proteomics boom.
Key Features:
* focuses on gene discovery, genomics, and DNA array technology
* covers quantitative PCR techniques, including the use of standards and kinetic analysis
* includes statistical refinement of primer design parameters
* illustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, andin situ PCR

Edition details

• Publisher: Academic Pr
• Publication date: 1999
• ISBN 10: 0123721857
• ISBN 13: 9780123721853
• Binding: Hardcover
• Language: English
• Number of pages: 566
• Editor: Innis Michael A., Gelfand David H., Sninsky John J.