RNA Methodologies: A Laboratory Guide for Isolation and Characterization - Softcover

Farrell Jr., Robert E.

Softcover

ISBN 10: 0122497007 ISBN 13: 9780122497001

Publisher: Academic Press, 1993

Synopsis

This book is a collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA. It studies cellular regulation using RNA as a parameter of gene express, offers RNA isolation strategies, and explains proper handling, storage, and manipulation of RNA.

About this title

Review

Each of the protocols is written relatively independent of the rest of the text, allowing the reader to immediately begin using a procedure without having to study the text from cover to cover... Very few other molecular biology guide books are available that cover the isolation and manipulation of RNA as exhaustingly as does this one. This book would be highly valuable in any research facility engaged in molecular biology. --Mark A. Jandreski, Loyola University Medical Center, DOODY'S ELECTRONIC JOURNAL

From the Back Cover

The isolation of biologically competent and chemically stable RNA is central to molecular biology. There remains an academic imperative to unify the many complex facets of the characterizations of cellular biochemistry with particular regards to the use of RNA as a parameter of gene expression. This laboratory guide is a growing collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. The focus herein is how these methodologies can be used in the study of transcriptional- and post-transcriptional regulation of gene expression.

KEY FEATURES:
*New sections describing RNA isolation, subtraction-suppression PCR, high-throughput methods, bioinformatics, and RNAi
*Explains proper handling, storage, and manipulation of RNA
*An expanded section on RT-PCR, RACE, and related techniques
*Trouble-shooting options are incorporated throughout the text|The isolation of biologically competent and chemically stable RNA is central to molecular biology. There remains an academic imperative to unify the many complex facets of the characterizations of cellular biochemistry with particular regards to the use of RNA as a parameter of gene expression. This laboratory guide is a growing collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. The focus herein is how these methodologies can be used in the study of transcriptional- and post-transcriptional regulation of gene expression.

KEY FEATURES:
*New sections describing RNA isolation, subtraction-suppression PCR, high-throughput methods, bioinformatics, and RNAi
*Explains proper handling, storage, and manipulation of RNA
*An expanded section on RT-PCR, RACE, and related techniques
*Trouble-shooting options are incorporated throughout the text

Edition details

• Publisher: Academic Press
• Publication date: 1993
• Language: English
• ISBN 10: 0122497007
• ISBN 13: 9780122497001
• Binding: Plastic Comb
• Number of pages: 332