RNA Methodologies: A Laboratory Guide for Isolation and Characterization - Softcover

Farrell, Robert

Softcover

ISBN 10: 0122496957 ISBN 13: 9780122496950

Publisher: Academic Press Inc, 1998

Synopsis

This laboratory guide is a collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. This edition includes a variety of RNA-based PCR techniques, such as differential display PCR, and transcript quantification by competitive (mimic) PCR. The book focuses on how these methodologies can be used in the study of transcriptional and posttranscriptional regulation of eukaryotic genes. The liberal incorporation of flow charts, tables, and graphs facilitate learning and assist in the planning phases of projects. It is a valuable resource for investigators who wish to characterize cellular biochemistry.

About this title

Review

Each of the protocols is written relatively independent of the rest of the text, allowing the reader to immediately begin using a procedure without having to study the text from cover to cover... Very few other molecular biology guide books are available that cover the isolation and manipulation of RNA as exhaustingly as does this one. This book would be highly valuable in any research facility engaged in molecular biology. --Mark A. Jandreski, Loyola University Medical Center, DOODY'S ELECTRONIC JOURNAL

From the Back Cover

The isolation of biologically competent and chemically stable RNA is central to molecular biology. There remains an academic imperative to unify the many complex facets of the characterizations of cellular biochemistry with particular regards to the use of RNA as a parameter of gene expression. This laboratory guide is a growing collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. The focus herein is how these methodologies can be used in the study of transcriptional- and post-transcriptional regulation of gene expression.

KEY FEATURES:
*New sections describing RNA isolation, subtraction-suppression PCR, high-throughput methods, bioinformatics, and RNAi
*Explains proper handling, storage, and manipulation of RNA
*An expanded section on RT-PCR, RACE, and related techniques
*Trouble-shooting options are incorporated throughout the text|The isolation of biologically competent and chemically stable RNA is central to molecular biology. There remains an academic imperative to unify the many complex facets of the characterizations of cellular biochemistry with particular regards to the use of RNA as a parameter of gene expression. This laboratory guide is a growing collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. The focus herein is how these methodologies can be used in the study of transcriptional- and post-transcriptional regulation of gene expression.

KEY FEATURES:
*New sections describing RNA isolation, subtraction-suppression PCR, high-throughput methods, bioinformatics, and RNAi
*Explains proper handling, storage, and manipulation of RNA
*An expanded section on RT-PCR, RACE, and related techniques
*Trouble-shooting options are incorporated throughout the text

Edition details

• Publisher: Academic Press Inc
• Publication date: 1998
• Language: English
• ISBN 10: 0122496957
• ISBN 13: 9780122496950
• Binding: Paperback
• Edition number: 2
• Number of pages: 533