Guide to Molecular Cloning Techniques (Volume 152) (Methods in Enzymology, Volume 152) - Hardcover

Hardcover

ISBN 10: 012182053X ISBN 13: 9780121820534

Publisher: Academic Press, 1987

Synopsis

This self-contained, state-of-the-art manual is designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Available in an easy-to-use spiral-bound edition, Guide to Molecular Cloning Techniques progresses from basic techniques underlying much of recombinant DNA technology to a series of sections addressing commonly met problems.

Key Features* Comprehensive protocols compiled specifically for this volume* Among the topics covered:* Genomic cloning* Preparation and characterization of mRNAcDNA cloning* Screening libraries* Identification and characterization of clones* Methods written by 87 experts* Editors' notes, overviews, and extensive cross-referencing* Process Guide, a compendium of basic processes in molecular biology indexed for at-a-glance accessibility* A problem-solving quantitative approach* Emphasis on strategies for choosing alternative methods

About this title

Review

Praise for the Series
"The Methods in Enzymology series represents the gold-standard."
--NEUROSCIENCE
"Incomparably useful."
--ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
--BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
--CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
--ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists."
--JOURNAL OF CHROMATOGRAPHY

Edition details

• Publisher: Academic Press
• Publication date: 1987
• Language: English
• ISBN 10: 012182053X
• ISBN 13: 9780121820534
• Binding: Hardcover
• Number of pages: 812
• Editor: Abelson John N., Simon Melvin I., Berger Shelby L., Kimmel Alan R.