Histological and Histochemical Methods: Theory and Practice - Hardcover

Kiernan, John A.

Hardcover

ISBN 10: 0080362249 ISBN 13: 9780080362243

Publisher: Butterworth-Heinemann Ltd, 1990

Synopsis

This text presents the chemical and physical principles of fixation, staining and histochemistry. It assumes a basic level of chemical and biological knowledge, and requires little mathematical skill. The relations of chemical structures and reactions to fixation, tissue processing, staining, enzyme location, immunohistochemistry and other procedures are explained. For this edition the author has updated the text with the latest techniques and developments within the field, whilst retaining the details of the classic techniques still in use. The book offers a practical guide to the preparation of specimens for light microscopy and includes detailed practical instructions of the techniques used. This text presents the chemical and physical principles of fixation, staining and histochemistry. It assumes a basic level of chemical and biological knowledge, and requires little mathematical skill. The relations of chemical structures and reactions to fixation, tissue processing, staining, enzyme location, immunohistochemistry and other procedures are explained. For this edition the author has updated the text with the latest techniques and developments within the field, whilst retaining the details of the classic techniques still in use. The book offers a practical guide to the preparation of specimens for light microscopy and includes detailed practical instructions of the techniques used.

About this title

Review

'This book would serve as an excellent introduction for undergraduate and PhD students and will easily guide researchers and technicians in the comprehension of new techniques. The author emphasizes the importance of acquiring laboratory skills under the guidance of more experienced colleagues, which of course is true, but this book provides a wonderful introduction to the new researcher and provides alternatives to the more experienced colleague . This book is a must have for any laboratory using histological techniques. It is an excellent teaching resource for anyone new to the field and gives ideas to the more experienced histologist. This book is not for the bookshelf it should live on your laboratory bench.' --Journal of Anatomy

'I personally believe that this text should be found on the bookshelves of every histology and histochemistry laboratory as a basic reference source. It is not a book to be read from start to finish, but should be consulted by researchers and in particular students before undertaking histochemical staining procedures. The book is very reasonably priced and to be objective, the purchase price is less than the cost of one or two antibodies or most laboratory chemicals, which, unlike a book, have a very limited life.' --Acta Histochemica

'The book is written in a succinct manner and is very readable despite being a technical manual. Numerous diagrams on chemical structures and reactions with excellent legends also make this book very user-friendly. Besides providing the usual cookbook-type recipes for the various staining methods, the author also carefully explains the theory and biochemistry behind the methodology. This information, a valuable asset in troubleshooting, would be very helpful to students, histotechnologists, and even pathologists and researchers...the concise and well written text makes this book a very handy manual for all laboratories involved in histology and histochemistry.' --Journal of Histotechnology

'I personally believe that this text should be found on the bookshelves of every histology and histochemistry laboratory as a basic reference source. It is not a book to be read from start to finish, but should be consulted by researchers and in particular students before undertaking histochemical staining procedures. The book is very reasonably priced and to be objective, the purchase price is less than the cost of one or two antibodies or most laboratory chemicals, which, unlike a book, have a very limited life.' --Acta Histochemica

'The book is written in a succinct manner and is very readable despite being a technical manual. Numerous diagrams on chemical structures and reactions with excellent legends also make this book very user-friendly. Besides providing the usual cookbook-type recipes for the various staining methods, the author also carefully explains the theory and biochemistry behind the methodology. This information, a valuable asset in troubleshooting, would be very helpful to students, histotechnologists, and even pathologists and researchers...the concise and well written text makes this book a very handy manual for all laboratories involved in histology and histochemistry.' --Journal of Histotechnology

From the Publisher

The relations of chemical structures and reactions to fixation, tissue processing, staining, enzyme location, immunohistochemistry and other procedures are explained in simple, descriptive terms. As well as being a textbook, further references and more detailed information is available for those who wish to study a topic in more depth. For this new edition the author has updated the text with the latest techniques and developments within the field, whilst retaining the details of the classic techniques still in use.

Edition details

• Publisher: Butterworth-Heinemann Ltd
• Publication date: 1990
• ISBN 10: 0080362249
• ISBN 13: 9780080362243
• Binding: Hardcover
• Language: English
• Edition number: 2
• Number of pages: 425