Biotechnology: DNA to Protein -- A Laboratory Project in Molecular Biology - Softcover

Thiel, Teresa; Bissen, Shirley T.; Lyons, Eilene M.

Softcover

ISBN 10: 0072416645 ISBN 13: 9780072416640

Publisher: McGraw-Hill Higher Education, 2001

Synopsis

This one-semester, project-based laboratory manual gives junior/senior level students the opportunity to characterize the enzyme alpha-amylase. As students proceed through the sequenced experiments, they will learn the principles of DNA, RNA, and protein structure by using modern-day laboratory techniques. Genetics, cell biology, and organic chemistry are prerequisites.

About this title

From the Publisher

Students will study two important aspects of biotechnology using the alpha-amylase gene and alpha-amylase protein: 1) the nature of proteins and their action, and 2) the principles and methods for cloning and expressing a gene. Therefore, the analysis of protein and the analysis of DNA are integrated within a single project.
The computer modeling software, RASMOL, is used in several lab exercises to study the three-dimensional structure of the alpha-amylase protein. Exercises in Appendix I will introduce students to some bioinformatics techniques that use sequence databases as well as sequence analysis software.
Many of the techniques that are used at the beginning of the project will be repeated throughout the project. These recurring techniques will not only reinforce students' learning but will also show students that techniques can be used in different contexts to give different information.
This project gives students an opportunity to apply biotechnology to a real, modern-day project. Not only will students need to evaluate the project in terms of the molecular biology but also in terms of practical considerations such as production and cost.

Edition details

• Publisher: McGraw-Hill Higher Education
• Publication date: 2001
• Language: English
• ISBN 10: 0072416645
• ISBN 13: 9780072416640
• Binding: Spiral-bound
• Number of pages: 192