Heterochromatin Protein 1? (HP1?): It's role on genomic stability and cellular senescence - Softcover

BILLUR, MUSTAFA

Softcover

ISBN 10: 3838123190 ISBN 13: 9783838123196

Publisher: Suedwestdeutscher Verlag fuer Hochschulschriften, 2011

Synopsis

Heterochromatin protein 1 (HP1) proteins are fundamental units of heterochromatin packaging.In mammals,there are three HP1 homologues termed HP1?(Cbx5),HP1?(Cbx1) and HP1?(Cbx3).HP1? is the best characterized isoform.Murine HP1? is essential for organismal survival.This study demonstrates that Cbx1+/- and Cbx1-/- MEFs escape senescence crisis that is associated with chromosomal aberrations.Telomeres of late passage Cbx1-/- MEFs are longer than WT controls. Introduction of Cbx1-/- mutation in cells expressing H-rasV12 oncogene did not result senescence bypass.K-rasV12 oncogene expression in Cbx1+/- mice resulted in increased malignant adenocarcinomas.These data indicate that HP1? acts as a tumour suppressor.Other experiments showed that binding of HP1? to histone H3(HH3) is resistant to high salt concentrations.ITC experiments confirmed that binding affinity of HP1? for HH3 was 4 times higher than for H3K9me3.The high affinity binding of HP1? to the HH3 is shown to be stronger than the affinity to H3K9me3.Loss of this interaction might result in the perinatal lethal phenotype seen in Cbx1-/- mice.

About this title

About the Author:

Dr.Billur studied biology at the Ege University in Turkey.He continued his studies with Prof.Billett at the NTU in Nottingham, UK where he received his MSc degree.He then joined the Singh lab at Research center Borstel in Germany where he worked on heterochromatin protein 1.He successfully defended his PhD thesis in 2010 at the CAU in Germany.

About this edition

• Publisher: Suedwestdeutscher Verlag fuer Hochschulschriften
• Publication date: 2011
• ISBN 10: 3838123190
• ISBN 13: 9783838123196
• Binding: Paperback
• Number of pages: 160