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Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973584ISBN 13: 9781493973583
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This concise and practical resource brings together recent advances in identifying and managing anemia of chronic disease (inflammation), genetically related anemia and anemia related to chronic end organ damage. Chapters provide adetailed analysis of the current science of anemia, approaches to different patient populations, comorbid conditions and nutritional aspects of anemia. Novel therapies focused on physiological pathways are introduced and discussed.Controversies from the perspective of subspecialists focused in treating major causes of anemia within their specific disciplines are also presented. Easy-to-reference and authored by experts in each clinical scenario, Management of Anemia is the launching point for learning more about this challenging and common condition. 256 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973320ISBN 13: 9781493973323
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The new edition of this volume features a concise yet comprehensive review of sepsis. In addition to thoroughly revised chapters from the previous edition that covers the latest in diagnostics, therapeutics, and antimicrobials, the third edition features new chapters on the economic impact of infections and antibiotics, multi-organ failure in sepsis, and current strategies for training high-performance teams.Written by an international group of experts in the field, Sepsis, Third Edition is a valuable resource that provides the most practical information for clinicians and practitioners who treat this complication in the ICU. 184 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973614ISBN 13: 9781493973613
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides readers with the latest techniques and approaches used to study K+ channels. There are a wide range of topics covered in the book, such as channel structure; K+ channels and their roles in physiology and disease; single-molecule FRET; proteins expression levels in vivo; real-time correlation of channel activity; gating; pharmacology; physiological functions; and channelopathies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Potassium Channels: Methods and Protocols is a valuable resource for all researchers who are interested in studying potassium channels. 364 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973827ISBN 13: 9781493973828
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details protocols that broadly cover many aspects of basic and translational research onBorrelia burgdorferi.Chapters guide readers through epidemiology and ecology, cultivation, cell structure, physiology, genomics and transcriptomics, proteomics, animal infection, pathogenesis and host responses, and vaccines. These essential protocols incorporate the most recent, practical, and innovative research tools aiding new and experienced researchers in their studies involving the biology, pathogenesis, and prevention ofB. burgdorferiinfection. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge,Borrelia burgdorferi: Methods and Protocolsaims to ensure successful results in the further study of this vital field of biomedical research. 376 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493974335ISBN 13: 9781493974337
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field 236 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 149397338XISBN 13: 9781493973385
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume covers methods that analyze various Argonaute proteins from a variety of organisms to help researchers better understand their properties ranging from a molecular level to an organismal level. The chapters in this book explore the following topics: identification and expression analysis of guide nucleic acids and their targets; analysis of biochemical properties of Argonautes; biological functions of Argonautes; and obtaining materials and setting up analysis platforms. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative,Argonaute Proteins: Methods and Protocolsis a valuable resource for researchers and scientists looking to expand their knowledge of Argonaute proteins and their functions. 276 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973703ISBN 13: 9781493973705
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative medicine in the future. The chapters in this book were designed to address cellular quiescence in prokaryote and eukaryote organisms, detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU, H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal, neuronal, hematopoietic), genomic regulation (gene expression, transcription factors, lncRNA, RNA methylation), as well as analysis of the heterogeneity of quiescence by computer modeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cellular Quiescence: Methods and Protocols offers a broad view of basic and cutting-edge technology to inspire research in this emerging field of cell biology. 316 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493972766ISBN 13: 9781493972760
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents protocols for Brachypodium genomics in numerous areas ranging from marker development, trait evolution, functional genomics, metabolomics, transcriptomics, genomics, and tilling. This book also explores techniques to study the widening genetic base of Brachypodium that will help researchers better understand the model plant using NGS technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Brachypodium Genomics: Methods and Protocols is a valuable resource for bench-oriented molecular biologists and computational biologists working towards further evolving this field. 328 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973886ISBN 13: 9781493973880
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents a range of different techniques that have been used to characterize the structure and function of the endoplasmic reticulum (ER) in higher plants. Chapters guide readers through application of modern microscopy techniques by fluorescence and electron microscopy, new protocols for analysing ER network structure, methods to purify and analyse ER membrane structure and to study protein glycosylation, protocols to study the unfolded protein response, and the role of the ER in autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Plant Endoplasmic Reticulum: Methods and Protocols aims to ensure successful results in the further study of this vital field. 280 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493972731ISBN 13: 9781493972739
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The aim of this volume is to make computer programs for analyzing human genetic data more easily accessible to the beginner.Statistical Human Genetics: Methods and Protocols, Second Edition provides updated and new chapters detailing genetic terms, analysis software, and how to interpret the program outputs. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, step-by-step instructions, and tips on troubleshooting and avoiding known pitfalls. The purpose of Statistical Human Genetics: Methods and Protocols, Second Edition is to ensure successful and meaningful results in the fast-growing field of genetic epidemiology. 704 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493974882ISBN 13: 9781493974887
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This ambitious volume brings together and assesses all major systematic reviews of the effectiveness of criminological interventions, to draw broad conclusions about what works in policing, corrections, developmental prevention, situational prevention, drug abuse treatments, sentencing and deterrence, and communities.Systematic reviews aim to minimize any possible bias in drawing conclusions by stating explicit criteria for inclusion and exclusion of studies, by conducting extensive and wide-ranging searches for possibly eligible studies, and by making all stages of the review explicit and transparent so that the methods can be checked and replicated. Over a decade ago, a concerted effort was made by members of the criminology community, including the Editors and contributors of this volume, to bring the practice of systematic reviews to the study of Criminology, providing replicable, evidence-based data to answer key questions about the study of crime causation, detection, and prevention. Now, the pioneers in this effort present a comprehensive stock-taking of what has been learned in the past decade of systematic reviews in criminology. Much has been discovered about the effectiveness of (for example) boot camps, 'hot spots' policing, closed-circuit television surveillance, neighborhood watch, anti-bullying programs in schools, early parenting programs, drug treatment programs, and other key topics.This volume will be of interest to researchers in criminology and criminal justice, as well as in related fields such as public health and forensic science, with important implications for policy-makers and practitioners.Decisively showing that the 'nothing works' era is over, this volume takes stock of what we know, and still need to know, to prevent crime. I plan to keep this book close at hand and to use it often!Francis T. Cullen, Distinguished Research Professor Emeritus, University of CincinnatiAta time when there is a broad commitment to bringing science to the front lines of practice, this book should be on the reading list of both policymakers and scholars.Laurie O. Robinson, Clarence J. Robinson Professor of Criminology, Law Society, George Mason University and former Assistant Attorney General of the U.S. Department of Justice 352 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973649ISBN 13: 9781493973644
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 364 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493974009ISBN 13: 9781493974009
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. These include surface marker expression, side population, spheres formation, ALDH activity, and numerous others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert tips and troubleshooting advice to avoid potential pitfalls. Comprehensive and practical, Cancer Stem Cells: Methods and Protocols provides researchers with the techniques they will need in order to pursue vital research into this complex field of study. 240 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 149397355XISBN 13: 9781493973552
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. Neuware -This second edition details some of the recent trends in HCS/HCA/HCI. New and updated chapters guide readers through methods that utilize reagents and kits that have been developed to measure cells and subpopulation classifications, control measures, overviews of the data handling issues associated with HCS/HCA/HCI, and methods to implement more complex phenotypic models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High Content Screening and Analysis-The Ideal Format for Phenotypic Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery, Second Edition aims to ensure successful results in the further study of this vital field. 416 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493974092ISBN 13: 9781493974092
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides comprehensive and detailed protocols that discuss proteomic techniques, plant endosomes, and isolation of organelles and subcellular fractions. The chapters in this book explore numerous plant species and cover topics, such as isolation and purity assessment of membranes from Norway spruce; proteomic analysis of nuclei; analyzing the vacuolar membrane (tonoplast) proteome; isoforms of a thylakoid-bound protein; assay of plasma membrane H+-ATPase in plant tissue under abiotic stresses; and identification and characterization of plant membrane proteins using ARAMEMNON. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and thorough, Plant Membrane Proteomics: Methods and Protocols is a valuable resource that promotes the use of plant membrane proteomics to develop the future of the field. 292 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973207ISBN 13: 9781493973200
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest techniques used by researchers to help them better understand the NMDAR structure-function relations, principles, and rules that govern how NMDARs operate in brain processing. The volume includes a detailed introductory chapter describing the field and is divided into three parts. Topics covered in the volume include: quantification of NMDAR subunit genes expression by qRT-PCR; detection of NMDARs antibodies in encephalitis; recombinant channels in host cells using a fast agonist application system; GluNs detection and functions in microglial cells; and NMDARs as voltage sensors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, NMDA Receptors: Methods and Protocols is a valuable resource that discusses the diversity of the currently-used methods, the importance of NMDARs and their complexity, and the progress that has already been made. This book will be of interest to scientists, clinicians, and industry professionals working in this field. 328 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973797ISBN 13: 9781493973798
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This up-to-date volume includes protocols that illustrate the broad use of chromatin immunoprecipitation (ChIP) and ChIP-related methods in a variety of biological research areas. The collection also includes protocols designed to improve the performance of ChIP for specific applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chromatin Immunoprecipitation: Methods and Protocols features techniques, including bioinformatic analysis of ChIP data, will be of interest to a very broad research community in the fields of biochemistry, molecular biology, microbiology, and biomedicine. 256 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493974246ISBN 13: 9781493974245
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents a broad compendium of techniques used in biodegradation and toxicological research. Through both legacy and up-to-date approaches to practical methodologies with proven results, the book examines the role and applications of analytical biodegradation quantification as it applies to the environmental sciences, particularly in the range of byproducts that are usually linked to toxicology, and the test organisms most often used in toxicity testing. Topics include scientific and technical feasibility studies, contaminant impacts evaluation, study design and analytical techniques, key methodologies required to prepare the biodegradation and toxicology protocols, as well as the handling of microbial communities related to such processes. Written for the Methods in Pharmacology and Toxicology series, chapters deal with a critical discussion of laboratory scale experiments, as well as full scale in situ and ex situ apparatus, with each chapter containing both a discursive section along with a detailed methods section. Detailed and authoritative, Toxicity and Biodegradation Testing is primarily focused toward the environmental sciences researcher, while the range of techniques also provides an introduction to biodegradation and toxicology methods for researchers outside of the field. 412 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1441902023ISBN 13: 9781441902023
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This innovative collection offers a wide-ranging palette of psychological, public health, and sociopolitical approaches toward addressing the multi-level prevention needs of gay men living with HIV and AIDS. This book advances our understanding of comprehensive health care, risk and preventive behaviors, sources of mental distress and resilience, treatment adherence, and the experiences of gay men's communities such as communities of color, youth, faith communities, and the house ball community. Interventions span biomedical, behavioral, structural, and technological approaches toward critical goals, including bolstering the immune system, promoting safer sexual practices, reducing HIV-related stigma and discrimination, and eliminating barriers to care. The emphasis throughout these diverse chapters is on evidence-based, client-centered practice, coordination of care, and inclusive, culturally responsive services. Included in the coverage: Comprehensive primary health care for HIV positive gay men From pathology to resiliency: understanding the mental health of HIV positive gay men Emerging and innovative prevention strategies for HIV positive gay men Understanding the developmental and psychosocial needs of HIV positive gay adolescent males Social networks of HIV positive gay men: their role and importance in HIV prevention HIV positive gay men, health care, legal rights, and policy issues Understanding Prevention for HIV Positive Gay Men will interest academics, researchers, prevention experts, practitioners, and policymakers in public health. It will also be important to research organizations, nonprofit organizations, and clinical agencies, as well as graduate programs related to public health, consultation, and advocacy. 448 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493972677ISBN 13: 9781493972678
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides a comprehensive study of this key method of gel-based proteomics. Chapters in Difference Gel Electrophoresis: Methods and Protocols detail methods on principles of differential protein labeling, two-dimensional gel electrophoresis, techniques on optimized proteomic workflows, advanced mass spectrometry for protein identification, application of basic biological research, and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field. 328 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973509ISBN 13: 9781493973507
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition volume expands on the first edition by providing up-to-date protocols to characterize nanomaterials used as drug delivery agents. The chapters in this book are divided into 5 parts and cover topics such as: advances and obstacles in nanomedicine research; methods to test sterility and endotoxin, physicochemical features, immunological effects, drug release, and in vivo efficacy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Characterization of Nanoparticles Intended for Drug Delivery, Second Edition is a valuable tool for researchers and pharmaceutical and biotechnology developers who are evaluating the clinical potential of nanomedicines in preclinical studies. 268 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973142ISBN 13: 9781493973149
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume discusses techniques used for the molecular characterization of maize. This book is divided into 4 parts: cell, tissue, and organ culture and maize transformation; gene silencing and generation of mutant populations; plant gene expression; and plant metabolic networks. The chapters cover a range of topics, such as growing and propagating maize in the laboratory, greenhouse and field studies, screening mutagenic population, characterizing the genome, describing protein and metabolic regulatory networks, and generating transgenic plants for gene knock-out and over expression purposes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Maize: Methods and Protocols is a valuable resource for everyone who is interested in maize research. 248 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493974157ISBN 13: 9781493974153
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume focuses on the optogenetics workflow, and covers topics on viral vectors, targeting strategies, choice on opsins, animal models and readouts, and applications in systems neuroscience. This book shows readers how to identify the critical aspects of each methodological step, and how to determine the necessary level of complexity to address the particular research question. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Comprehensive and cutting-edge, Optogenetics: A Roadmap is a valuable guide for both the optogenetics novices and the experts. 316 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973738ISBN 13: 9781493973736
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores experimental approaches used to study Duchenne muscular dystrophy (DMD), an X-linked degenerative skeletal muscle disease caused by mutations in the dystrophin gene. Including the latest progress and scientific achievements, the book covers recent discoveries achieved through in vivo gene editing which have proven to be promising in restoring dystrophin expression, at least in ameliorating skeletal muscle symptoms, and the contents focus on 'Omics' techniques in gene expression, protein expression, miRNAs, and long non-coding RNA analysis, as well as experimental studies of the structural/functional changes affecting the skeletal and cardiac muscles and ongoing preclinical studies and clinical trials. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Duchenne Muscular Dystrophy: Methods and Protocols serves as a guide for researchers exploring the complicated nature of dystrophin in the hope of helping the victims of this disorder. 304 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973053ISBN 13: 9781493973057
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents forty-two methods and protocols to analyze diverse aspects of genome instability. Chapters detail mutagenesis and repair, methods to quantify and analyze the properties of DNA double-strand breaks, profile replication, replication proteins strand-specifically, genome instability, fluorescence microscopic techniques, and genomic and proteomic approaches. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge,Genome Instability: Methods and Protocolsaims to provide a comprehensive resource for the discovery and analysis of the proteins and pathways that are critical for stable maintenance of the genome. 684 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493972308ISBN 13: 9781493972302
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Reflecting developments in genome editing, this third edition volume fully updates a collection of key techniques for the study of functional genomics. The book is broken up into sections on bioinformatics, DNA, RNA, and protein analysis, as well as a closing section entitled 'From Genotype to Phenotype.' Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Updated and authoritative, Functional Genomics: Methods and Protocols, Third Edition seeks to aid scientists in establishing or extending technologies and techniques in their laboratories. 408 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493969803ISBN 13: 9781493969807
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents protocols that analyze and explore hemorrhagic fever viruses (HFV). This book is divided into 5 parts: Part I begins with an overview on predicting viral pandemics and then covers methods for surveillance, diagnosis, and classification of HFV. This includes an antibody capture method using Lassa virus antigens. Part II discusses structural studies and reverse genetics of HFV. The chapters in this part describe envelope glycoprotein membrane fusion studies, arenavirus nucleocapsid protein, and the use of virus-like-particles to study viral egress. Part III explores in vivo models of HFV infections, and contains chapters on murine, guinea pig, and primate models for HFV, and methods to obtain a subset of primary human liver cells that can be cultured long-term. Part IV looks into immune assays and vaccine production for HFV. The chapters in this section cover the attenuated vaccine for Argentine HFV, detecting virus-antibody immune complexes in secondary dengue infections, and DNA vaccination. Part V discusses host responses to viral hemorrhagic fever, and contains chapters on identifying host restrictions to Junín or Dengue infection, and a cell-culture method to assess coagulation after HFV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Hemorrhagic Fever Viruses: Methods and Protocols is a valuable resource for scientists and researchers who want to bridge the gap between virus recognition in surveillance and understanding host responses to infection. 444 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493974270ISBN 13: 9781493974276
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells. Protocols include strategies to analyze circulating red blood cell parameters, as well as progenitor cell status, stage of differentiation, and enucleation. Methods for the study of erythroid cell gene regulation using chromatin immunoprecipitation and chromatin conformation capture are detailed. Furthermore, protocols are provided to examine stress erythropoiesis, erythroblastic islands, and erythroblast structure. The collection also includes reviews on the available mouse models of erythropoiesis, as well as on future considerations for good manufacturing practice in order to translate the manufacture of erythrocytes to the clinic. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Erythropoiesis: Methods and Protocols provides a definitive source for molecular and cellular biology protocols to study erythropoiesis and to move this vital field forward. 312 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973444ISBN 13: 9781493973446
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fourth edition volume expands on the previous editions by presenting readers with new and emerging methodologies in cytometry. The chapters in this book cover various topics such as quantifying surface and intracellular protein biomarkers, intracellular cytokine staining, apoptosis analysis, cell cycle analysis, tracking cell proliferation, and monitoring protein-protein interactions using FRET. Some developing methodologies that explored are mass cytometry, vesicle flow cytometry, time-resolved flow cytometry, and real-time label-free deformability cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Flow Cytometry Protocols, Fourth Edition researchers and scientists who are interested in continuing or expanding their knowledge of flow cytometry. 508 pp. Englisch.
Published by Springer New York Okt 2017, 2017
ISBN 10: 1493973975ISBN 13: 9781493973972
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides readers with a collection of the latest protocols used by researchers to study polyamines (PA). The chapters in this book cover various topics, such as quantification of different polyamines and conjugates, subcellular localization studies, transport, DNA methylation, ODC regulation, genetic and phenotyping analyses, genome-wide association mapping, polyamine applications and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Polyamines: Methods and Protocols is a useful reference for researchers looking to advance and stimulate their knowledge of polyamines. 532 pp. Englisch.