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Autoimmune diseases occur when physiological tolerance to self-antigens is lost and thereafter, human body can not discriminate between self and non-self tissue. In autoimmunity, production of different auto-antibodies result in variety of pathologic manifestations. Auto-antibodies are hallmark of both systematic and organ specific autoimmune diseases. Among various autoantibodies, anti-neutrophil cytoplasmic antibody (ANCA) is a useful diagnostic tool for auto-immune glomerulonephritis. There are many techniques used for the detection of ANCA level. Here, we want to introduce some common techniques i.e. ELISA, IFT, cytogram and flow cytometer. Objectives: Current study was designed to determine sensitivity & specificity ANCA (p-ANCA and c-ANCA) of ELISA method. Results: ELISA is most sensitive method for c-ANCA and most specific method for p-ANCA. Diagnostic value of ELISA for both parameters c-ANCA & p-ANCA is same 95.31%. Conclusion: ELISA is sensitive as well as specific method for the detection of ANCA. Besides that indirect immunoflorecent technique (IIF) remains the gold standard method for the detection of ANCA (c-ANCA & p-ANCA).
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Maria Arif, MPhil, Biochemistry and Molecular Biology, NUST. Isl. PAK, working as Medical Technologist at NUST, Isb, PAK. I have completed my post graduation from UHS, Lhr, PAK in field of Lab Technology in session 2007-2009. I have obtained scholarship from HEC.PAK throughout my post graduation period. I was graduated from PU, Lhr, PAK.
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- PublisherLAP LAMBERT Academic Publishing
- Publication date2012
- ISBN 10 3659120057
- ISBN 13 9783659120053
- BindingPaperback
- Number of pages68
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Book Description Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Autoimmune diseases occur when physiological tolerance to self-antigens is lost and thereafter, human body can not discriminate between self and non-self tissue. In autoimmunity, production of different auto-antibodies result in variety of pathologic manifestations. Auto-antibodies are hallmark of both systematic and organ specific autoimmune diseases. Among various autoantibodies, anti-neutrophil cytoplasmic antibody (ANCA) is a useful diagnostic tool for auto-immune glomerulonephritis. There are many techniques used for the detection of ANCA level. Here, we want to introduce some common techniques i.e. ELISA, IFT, cytogram and flow cytometer. Objectives: Current study was designed to determine sensitivity & specificity ANCA (p-ANCA and c-ANCA) of ELISA method. Results: ELISA is most sensitive method for c-ANCA and most specific method for p-ANCA. Diagnostic value of ELISA for both parameters c-ANCA & p-ANCA is same 95.31%. Conclusion: ELISA is sensitive as well as specific method for the detection of ANCA. Besides that indirect immunoflorecent technique (IIF) remains the gold standard method for the detection of ANCA (c-ANCA & p-ANCA). 68 pp. Englisch. Seller Inventory # 9783659120053
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Book Description Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Autoimmune diseases occur when physiological tolerance to self-antigens is lost and thereafter, human body can not discriminate between self and non-self tissue. In autoimmunity, production of different auto-antibodies result in variety of pathologic manifestations. Auto-antibodies are hallmark of both systematic and organ specific autoimmune diseases. Among various autoantibodies, anti-neutrophil cytoplasmic antibody (ANCA) is a useful diagnostic tool for auto-immune glomerulonephritis. There are many techniques used for the detection of ANCA level. Here, we want to introduce some common techniques i.e. ELISA, IFT, cytogram and flow cytometer. Objectives: Current study was designed to determine sensitivity & specificity ANCA (p-ANCA and c-ANCA) of ELISA method. Results: ELISA is most sensitive method for c-ANCA and most specific method for p-ANCA. Diagnostic value of ELISA for both parameters c-ANCA & p-ANCA is same 95.31%. Conclusion: ELISA is sensitive as well as specific method for the detection of ANCA. Besides that indirect immunoflorecent technique (IIF) remains the gold standard method for the detection of ANCA (c-ANCA & p-ANCA). Seller Inventory # 9783659120053
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Book Description Kartoniert / Broschiert. Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Arif MariaMaria Arif, MPhil, Biochemistry and Molecular Biology, NUST. Isl. PAK, working as Medical Technologist at NUST, Isb, PAK. I have completed my post graduation from UHS, Lhr, PAK in field of Lab Technology in session 2007-200. Seller Inventory # 5132766
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