Development of a simple, reproducible and efficient in-vitro culture protocol for callus proliferation and direct organogenesis from nodal explants of Scoparia dulcis Linn, an exotic medicinal plant - Softcover

Vazhacharickal, Prem Jose; Mathew, Noby; P. R, Padmasree

9781973353829: Development of a simple, reproducible and efficient in-vitro culture protocol for callus proliferation and direct organogenesis from nodal explants of Scoparia dulcis Linn, an exotic medicinal plant

Softcover

ISBN 10: 1973353822 ISBN 13: 9781973353829

Publisher: Independently published, 2017

For the development of an efficient callus initiation and direct organogenesis protocol in Scoparia dulcis Linn., an important medicinal plant, nodal segments of field grown plants were aseptically cultured on agar solidified MS medium supplemented with different concentrations and combinations of three PGRs, namely IAA, NAA and BA. The explants produced a green or light green compact callus on MS medium fortified with 0.5-2.0 mg/L BA in combination with 0.5-1.0 mg/l IAA or NAA. The maximum amount of callus was produced on MS with 1.5 mg/l BAP + 0.5 mg/l NAA in the case of explants. These callus tissues underwent differentiation when grown on a range of PGRs (BAP, IAA and NAA) supplemented media. The maximum number of callus was developed in medium containing 1.5 mg/l BAP + 0.5 mg/l NAA. Direct organogenesis underwent rapid elongation on elongation media and maximum elongation took place on MS with 1.5 mg/l BAP + 0.5 mg/l IAA. Antimicrobial activity of ethanol extracts of S. dulcis was also determined by using various bacterial strains. Ethanolic extracts of S. dulcis showed moderate activity against various organisms.

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