Heterologous Expression of Membrane Proteins: Methods and Protocols: 601 (Methods in Molecular Biology, 601) - Softcover

Softcover

ISBN 10: 1493961322 ISBN 13: 9781493961320

Publisher: Humana, 2016

Synopsis

This book provides an overview of the different heterologous expression systems available to produce various membrane proteins for structural analysis. Chapters examine membrane protein solubilization, purification, and instability in solution.

About this title

Review:

From the reviews:

“Well-illustrated book provides an overview of different heterologous expression systems available to produce membrane proteins for structural analysis to facilitate understanding of how these proteins function and how their functions can be modified by drug treatment. ... Written for students and experienced researchers, the book also will be useful for clinicians interested in the care of patients with cancer ... . provides valuable information on membrane proteins for novice and experienced researchers. Clinicians will find it extremely useful for developing methods and protocols for research.” (Omer Iqbal, Doody’s Review Service, July, 2010)

From the Back Cover:

Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field. 

About this edition

• Publisher: Humana
• Publication date: 2016
• ISBN 10: 1493961322
• ISBN 13: 9781493961320
• Binding: Paperback
• Number of pages: 283
• Editor: Mus-Veteau Isabelle