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"The book was written by the inventors of and leading experts in the technology and provides a comprehensive overview of what has been achieved with this relatively new approach. The detailed description of important experimental parameters and the protocols provided in this book make it a unique resource for all practitioners in the field and all scientists interested in the technology."--Analytical & Bioanalytical Chemistry, February 2014 "Boschetti and Righetti introduce the new technique of combinatorial peptide ligand libraries for identifying low-abundance peptide fragments. The standard proteomics techniques of mass spectrometry, 2D electrophoresis and chromatography are deliberately given brief treatment only as prefractionation tools in favor of new methodologies."--Reference & Research Book News, October 2013
Low-Abundance Proteome Discovery: State of the Art and Protocols addresses the biggest challenge in the field of protein biomarkers–the detection of low-abundance proteomes–by using an original strategy developed by the authors. This strategy, known as combinatorial peptide ligand libraries, permits the detection of species of proteins that are typically present in very low abundance and that may yield valuable clues to future discoveries.Low-Abundance Proteome Discovery: State of the Art and Protocols first presents an overview of low-abundance proteins and their importance for research and diagnostics, followed by a survey of conventional prefractionation methods in proteome analysis. Then, it describes the principles and applications of peptide ligand libraries as a unique approach for the detection of low-abundance proteins. Finally, it offers a detailed description of laboratory practices and recipes that will help you succeed in detecting and identifying low-abundance proteins in various situations.
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