Neurons: Methods and Applications for the Cell Biologist,71: Volume 71 (Methods in Cell Biology, Volume 71) - Softcover

Peter J. Hollenbeck

9780123525666: Neurons: Methods and Applications for the Cell Biologist,71: Volume 71 (Methods in Cell Biology, Volume 71)

Softcover

ISBN 10: 0123525667 ISBN 13: 9780123525666

Publisher: Academic Press, 2003

Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented.

Contains culture methodology for more than a dozen types of CNS and PNS neurons
Includes most recent and reliable techniques from expert practitioners for specific experimental applications
Addresses the latest strategies for transfecting neurons

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Review:

Teaches cell biologists the proper techniques to grow nerve cells as they are well-suited to almost every area of cell biological inquiry.

From the Back Cover:

Written by cell biologists and neuroscientists, this volume in the Methods in Cell Biology series is a detailed guide for cell biologists who would like to approach their experimental questions using nature's most interesting cells - neurons. Many cell biologists study the properties of cells grown outside of the animal in the controlled environment of a culture dish, but they would benefit from directing their attention to nerve cells as they are well-suited to almost every area of cell biological inquiry. However, many are afraid to try because neurons have the reputation of being difficult and unreliable compared to other kinds of cells.

The first section lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. The second section contains chapters that explain recent advances in our ability to introduce exogenous proteins and genes into neurons in culture. Also discussed in this section is the development of procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons.

With the current and reliable information contained in this volume, growing various kinds of neurons in culture and using them for diverse experimental applications will be easy for cell biologists to master!

Key Features:
* Contains culture methodology for more than a dozen types of CNS and PNS neurons
* Discusses the latest strategies for transfecting neurons
* Presents, in 19 chapters, the most current and robust techniques for specific experimental applications

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