Introduction to Electron Microscopy for Biologists: Methods in Cell Biology: 88: Volume 88 (Methods in Cell Biology, Volume 88) - Hardcover

Terry D. Allen

9780123743206: Introduction to Electron Microscopy for Biologists: Methods in Cell Biology: 88: Volume 88 (Methods in Cell Biology, Volume 88)

Hardcover

ISBN 10: 0123743206 ISBN 13: 9780123743206

Publisher: Academic Press, 2008

This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers).
*Basic techniques in transmission and scanning electron microscopy
*Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton
*Discussion on electron microscopy of viruses and virus-cell interactions

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Review:

This volume demonstrates how electron microscopy contributes to knowledge about biological structural information.

Synopsis:

"Introduction to Electron Microscopy for Biologists" is ideal for the scientist who may be considering electron microscopy as a tool to extend molecular, biochemical, or light microscope observations to the next level of structural information, only available by electron microscopy. Each chapter briefly surveys the present state of structural information in a particular area, be it an individual but widely occurring molecule such as actin or collagen, together with the methods for visualization, either as an extracted and purified entity, or in situ within its biological context. Not only is this book an introduction to electron microscopy in general, but it is also useful for those within the field who wish to move to a different area of expertise, for instance an approach based on rapid freezing, rather than more conventional protocols.This should be a first choice reference for any biologist wanting to know what does it look like across the full spectrum of cell and molecular biology of life science.

It illustrates the advantages of electron microscopy and the protocols required for fine structural visualization of individual molecules upwards, ranging from mammalian tissues to experimental organisms. It includes comprehensive chapters that cover both structural information on each area as well as the protocols themselves. It includes methods covering specific labeling, as well as methods for quantification and three dimensional reconstruction from molecular to tissue levels.

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