Adenylyl Cyclase, G Proteins, and Guanylyl Cyclase: 195 (Methods in Enzymology): Volume 195 - Hardcover

Hardcover

ISBN 10: 0121820963 ISBN 13: 9780121820961

Publisher: Academic Press, 1991

Synopsis

This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases.

• Adenylyl cyclase: determination of activity
• Preparation of materials useful in purification of components of hormonally responsive cyclase systems
• Purification guanine nucleotide-dependent regulatory proteins
• Purification and characterization of g proteins: labeling and quantitating of G proteins
• Reconstitution guanylyl cyclase: assay
• Preparation of materials used for study of the cyclase
• Purification
• Regulation

About this title

Review:

Praise for the Volume "Highly recommended. Methods books are an essential aid to the laboratory worker and this series has a well-deserved international reputation and requires no introduction. As usual, the book is very well presented and the list of contributing authors is extremely impressive." --NEUROSCIENCE

Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

"Incomparably useful." --ANALYTICAL BIOCHEMISTRY

"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY

"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY

"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS

"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA

"A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

Synopsis:

This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases. The key features include: adenylyl cyclase: determination of activity; preparation of materials useful in purification of components of hormonally responsive cyclase systems; purification guanine nucleotide-dependent regulatory proteins; purification and characterization of g proteins: labeling and quantitating of G proteins; reconstitution guanylyl cyclase: assay; preparation of materials used for study of the cyclase; purification; and, regulation.

About this edition

• Publisher: Academic Press
• Publication date: 1991
• ISBN 10: 0121820963
• ISBN 13: 9780121820961
• Binding: Hardcover
• Number of pages: 511
• Editor: Abelson John N., Simon Melvin I., Johnson Roger A., Corbin Jackie D.