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Enzymes: Biochemistry, Biotechnology, Clinical Chemistry

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9781904275275: Enzymes: Biochemistry, Biotechnology, Clinical Chemistry
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In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course.

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Review:

An essential prerequisite to explain structure and function of enzymes, and virtually every chapter tells of an advance in understanding proteins or their interactions. I will definitely use this book. --(Review of the first edition) Chemistry & Industry (Professor Carole Hackney, Keele University, UK)

About the Author:

Trevor Palmer was born in South Yorkshire and graduated from Cambridge University in 1966 with an honours degree in biochemistry, being influenced by Peter Sykes in organic chemistry and Malcolm Dixon in enzymology. He then worked as a clinical biochemist at the Queen Elizabeth Hospital for Children, linked to the Institute of Child Health, University of London, obtaining a PhD for research into inherited disorders. From this emerged the two main interests of his subsequent career, enzymology and evolution, the latter stimulating a further interest in catastrophism. He moved to Nottingham Trent University (then Trent Polytechnic) in 1974, initially as a lecturer in biochemistry, before becoming Head of Department of Life Sciences (1987), Dean of the Faculty of Science and Mathematics (1992), Senior Dean of the University (1998) and Pro Vice-Chancellor for Academic Development (2002), returning to predominantly academic activity as Emeritus Professor in 2006. His books include Understanding Enzymes (1981), Principles of Enzymology for Technological Applications (1993), Controversy - Catastrophism and Evolution (1999) and Perilous Planet Earth (2003). His wife, Jan, teaches psychology and sociology, and their children, James and Caroline, are currently at university carrying out postdoctoral and postgraduate studies, respectively.

Philip L. Bonner went to school in Coventry, West Midlands, before graduating from the University of Sussex in 1978 with an honours degree in Biochemistry. He then worked as a research assistant at Glaxo plc on Merseyside before leaving to take up a Research Assistant/Demonstrator post at Trent Polytechnic, where he obtained a PhD for research concerning enzymes associated with seed germination. Several postdoctoral appointments followed, at Bristol, Lancaster and Central Lancashire Universities, working on a variety of topics including relaxin, aspartate kinase and phospholipase C, before he was appointed as Senior Lecturer at Nottingham Trent University in 1991. There, he has maintained his research interests in enzymology and analytical biochemistry, working on the role of transglutaminase in plant/animal tissue and methods to isolate and characterise post-translationally-modified MHC peptides. His first single-author book, on protein purification, is due to be published in 2007. His wife, Liz, is a manager of an occupational therapist team in Nottingham and their daughter, Francesca, is at junior school.

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