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Research workers in biochemistry, cell biology, developmental biology, immunology, molecular biology, and neuroscience frequently need to examine cells or tissues microscopically. Unfortunately, many of them lack the appropriate education and technical training that are needed to use even the simplest microscope properly. The principles that govern the preparation of specimens also are commonly not known or badly misunderstood. This text informs research workers how to prepare cells and tissues for examination by traditional and modern types of light microscopy, and how to obtain and analyze images. The authors provide reasoned accounts of mechanisms and procedures, with selected references to original sources, review articles and textbooks. In addition, detailed technical instructions are provided for many important and frequently used methods. The theoretical background is also presented in sufficient detail to allow intelligent modification of these techniques to suit particular investigations to solve unexpected problems. Chapters are written by experts in their own fields (histotechnology or microscopy) for readers who are (or aspire to be) experts in biological or biochemical research. 2
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