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DNA Microarrays: A Molecular Cloning Manual

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9780879696245: DNA Microarrays: A Molecular Cloning Manual
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Designed to extend and complement the information in Molecular Cloning , this laboratory guide synthesizes the expertise and experience of about 30 innovators in DNA microarray technology, which is used to analyze genomes and characterize patterns of gene expression. The contributions emphasize the use of cDNA microarrays for expression analysis and focus upon two currently dominant technologies spotted DNA microarrays on glass slides, and in situ synthesized oligonucleotide arrays. Topics include generation of probes for spotted microarrays, printing spotted glass microarrays, expression analysis of RNA, membrane-based spotted cDNA arrays, tissue microdissection, using microarrays for genomic analysis, an introduction to microarray bioinformatics, and tissue microarrays. Edited by Botwell and Samrook, both of the Peter MacCallum Cancer Institute. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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DNA Microarrays: A Molecular Cloning Manual provides the reader with a wealth of information about how to manufacture microarrays, design and carry out microarray experiments, and analyze microarray data. The editors have lined up an impressive cast of authors who are in many cases the world experts on the particular aspect of microarrays technology that they describe... Overall, this is an excellent book, which will prove invaluable to any researcher involved in microarray analysis. It offers a comprehensive overview of the microarray technology as well as its satellite techniques, such as LCM, CGH arrays, and tissue arrays, and is the most comprehensive and up-to-date manual currently available on DNA microarrays. Journal of Proteome Research DNA Microarrays is a welcome addition to the library of anyone using microarrays. In the tradition of Cold Spring Harbor manuals, this volume provides detailed protocols for every aspect of the technology, from microarray fabrication to data analysis. The protocols cull the expertise of top microarray researchers and cover both expression profiling and analysis of genomic DNA... Overall, the protocols are comprehensive and sufficiently detailed for newcomers to microarrays, and the book contains enough pearls to ensure that it will also be welcomed in experienced microarrays labs. Nature Cell Biology

Synopsis:

DNA microarry, or DNA chip, technology is a new and powerful means of discovering, characterizing, and analyzing genes and their expression patterns. The potential of this technology is huge and its applications are widespread across many fields of biological and biomedical research. This manual assembles and synthesizes the expertise of over 30 innovators in this emerging field to provide detailed instruction on the design, construction, and applications of microarrays, as well as comprehensive descriptions of the software tools and strategies required for extensive image and data analysis.

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Publisher: Cold Spring Harbor Laboratory Pr..., 2002
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