Manipulating the Mouse Embryo: A Laboratory Manual, Fourth edition

Behringer, Richard; Gertsenstein, Marina; Nagy, Kristina; Nagy, Andras

Published by Cold Spring Harbor Laboratory Press
ISBN 10: 1936113007 / ISBN 13: 9781936113002
Used / Hardcover / Quantity Available: 0
Available From More Booksellers
View all  copies of this book

About the Book

We're sorry; this specific copy is no longer available. AbeBooks has millions of books. We've listed similar copies below.

Description:

1936113007 BRAND-NEW, Unread Copy in Perfect Condition. FAST FedEx shipping (you'll receive your order within 1-5 business days after shipping in most cases*), this helps to ensure your order arrives in perfect condition. PLEASE NOTE: FedEx does not generally deliver to PO Boxes or APO addresses, so please be sure to give us a physical street address to deliver to; also, unfortunately, we cannot ship this item to Alaska or Hawaii. THANKS! *(this applies to domestic shipments within the continental US - other destinations may take longer). Bookseller Inventory #

About this title:

Book ratings provided by GoodReads:
0 avg rating
(0 ratings)

Synopsis: This 4th edition of the "Mouse Manual"- Manipulating the Mouse Embryo-appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. While many of the techniques described in earlier editions of this manual have been relegated to core facilities, advances in DNA sequencing techniques and genome analysis have opened new avenues of research and developments. New approaches, such as the derivation of induced pluripotent stem cells and new targeted gene manipulation techniques that enable direct injection of RNA/DNA constructs into zygotes to achieve gene targeting, require new explanations and protocols. Authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy-pre-emininent leaders in their fields-have taken these developments into account in updating and rewriting this fourth edition to include new information and protocols on:

· generation of induced pluripotent stem cells
· RNA microinjections
· lentiviral microinjections and infection
· assisted reproduction techniques for sperm and embryo cryopreservation
· isolation, generation, and transplantation of spermatogonial stem cell lines
· in utero electroporation of gene constructs into postimplantation embryos
· vibratome sectioning of live and fixed tissues for imaging thick tissue sections
· whole-mount fluorescent staining methods for three-dimensional visualization

In addition, the wealth of essential information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups has been entirely updated. The first edition of Manipulating the Mouse Embryo appeared in 1986 as an outgrowth of Cold Spring Harbor Laboratory courses on the molecular embryology of the mouse held in the early 1980s, and authors of the first two editions included Brigid Hogan, Rosa Beddington, Frank Costantini, and Liz Lacy. The field's technological sophistication has grown exponentially since then, but the manual remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice.

I have the third edition. Why should I buy the fourth edition?
The new technologies regarding induced pluripotent stem cells and RNA injection and CRISPR technology are changing the field and opening up new avenues of investigation. The protocols for these as well as other up-to-the minute techniques in the fourth edition are by the leading proponents in the field and offer clear step-by-step guidance with copious illustrations.

To whom is this edition addressed-the neophyte or the experienced investigator?
Both groups can benefit from this manual. For the neophyte, the summary of mouse development and the how-to's of setting up a working laboratory are an invaluable introduction to the field. For the experienced investigator, the detailed protocols of new techniques in the field, with extensive troubleshooting and over-the-shoulder advice, allow easy integration of these techniques into a laboratory's research objectives.
Are the protocols in this book better than those available for download on the Web?
Many of the protocols available on the Web are perfectly good, but there are many that are not. Reagents are expensive and deadlines are tight. CSHL Press manuals provide protocols that have been formulated and tested in the labs of leading investigators in the field. They are reliable and they work. And they include the context and troubleshooting information that many online protocols do not have. In the case of the protocols in this manual, there is a bit of "art" required in the performance of these protocols. But the experience and advice of these authors provides all of the information needed to achieve success in the lab.

Product Description: This fourth edition of "The Mouse Manual¨is once again the definitive reference source on mouse development, transgenesis techniques, and molecular biology. Its preeminent authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy have reorganized and updated this edition to include new information and protocols on:



· generation of induced pluripotent stem cells


· RNA microinjections


· lentiviral microinjections and infection


· assisted reproduction techniques for sperm and embryo cryopreservation


· isolation, generation, and transplantation of spermatogonial stem cell lines


· in utero electroporation of gene constructs into postimplantation embryos


· vibratome sectioning of live and fixed tissues for imaging thick tissue sections


· whole-mount fluorescent staining methods for three-dimensional visualization

Recombinant DNA techniques and methods for studies of mouse embryonic development have been updated from previous editions, as has the wealth of information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups. The first edition of this classic work appeared more than 20 years ago, with authors that included Brigid Hogan, Rosa Beddington, Frank Costantini, and Elizabeth Lacy. The field's technological sophistication has grown exponentially but the manual remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice.

"About this title" may belong to another edition of this title.

Bibliographic Details

Title: Manipulating the Mouse Embryo: A Laboratory ...
Publisher: Cold Spring Harbor Laboratory Press
Binding: Hardcover
Book Condition: New

Top Search Results from the AbeBooks Marketplace

1.

Nagy
Published by Oxford University Press
ISBN 10: 1936113007 ISBN 13: 9781936113002
New Quantity Available: > 20
Seller
INDOO
(Avenel, NJ, U.S.A.)
Rating
[?]

Book Description Oxford University Press. Book Condition: New. Brand New. Bookseller Inventory # 1936113007

More Information About This Seller | Ask Bookseller a Question

Buy New
182.21
Convert Currency

Add to Basket

Shipping: 2.81
Within U.S.A.
Destination, Rates & Speeds

2.

Behringer, Richard; Marina Gertsenstein , Marina Gertsenstein ; Kristina Nagy , Kristina Nagy ; Andras Nagy, Andras Nagy
ISBN 10: 1936113007 ISBN 13: 9781936113002
New Quantity Available: 5
Seller
GreatBookPrices
(Columbia, MD, U.S.A.)
Rating
[?]

Book Description Book Condition: New. Bookseller Inventory # 19837256-n

More Information About This Seller | Ask Bookseller a Question

Buy New
264.63
Convert Currency

Add to Basket

Shipping: 2.12
Within U.S.A.
Destination, Rates & Speeds

3.

Behringer, Richard, Gertsenstein, Marina
Published by Cold Spring Harbor Laboratory (2013)
ISBN 10: 1936113007 ISBN 13: 9781936113002
Used Hardcover Quantity Available: 3
Seller
Murray Media
(North Miami Beach, FL, U.S.A.)
Rating
[?]

Book Description Cold Spring Harbor Laboratory, 2013. Hardcover. Book Condition: Very Good. Bookseller Inventory # P021936113007

More Information About This Seller | Ask Bookseller a Question

Buy Used
264.34
Convert Currency

Add to Basket

Shipping: 2.40
Within U.S.A.
Destination, Rates & Speeds

4.

Behringer, Richard, Gertsenstein, Marina
Published by Cold Spring Harbor Laboratory (2013)
ISBN 10: 1936113007 ISBN 13: 9781936113002
New Hardcover Quantity Available: 3
Seller
Murray Media
(North Miami Beach, FL, U.S.A.)
Rating
[?]

Book Description Cold Spring Harbor Laboratory, 2013. Hardcover. Book Condition: New. Bookseller Inventory # P111936113007

More Information About This Seller | Ask Bookseller a Question

Buy New
268.88
Convert Currency

Add to Basket

Shipping: 2.40
Within U.S.A.
Destination, Rates & Speeds

5.

Behringer, Richard; Gertsenstein, Marina; Nagy, Kristina; Nagy, Andras
Published by Cold Spring Harbor Laboratory Press (2013)
ISBN 10: 1936113007 ISBN 13: 9781936113002
New Hardcover Quantity Available: 1
Seller
Irish Booksellers
(Rumford, ME, U.S.A.)
Rating
[?]

Book Description Cold Spring Harbor Laboratory Press, 2013. Hardcover. Book Condition: New. book. Bookseller Inventory # 1936113007

More Information About This Seller | Ask Bookseller a Question

Buy New
279.38
Convert Currency

Add to Basket

Shipping: FREE
Within U.S.A.
Destination, Rates & Speeds

6.

Behringer, Richard/ Gertsenstein, Marina/ Nagy, Kristina/ Nagy, Andras
Published by Cold Spring Harbor Laboratory Pr (2013)
ISBN 10: 1936113007 ISBN 13: 9781936113002
New Hardcover Quantity Available: 1
Seller
Revaluation Books
(Exeter, United Kingdom)
Rating
[?]

Book Description Cold Spring Harbor Laboratory Pr, 2013. Hardcover. Book Condition: Brand New. 4th lab edition. 814 pages. 11.25x9.00x1.75 inches. In Stock. Bookseller Inventory # __1936113007

More Information About This Seller | Ask Bookseller a Question

Buy New
292.65
Convert Currency

Add to Basket

Shipping: 6
From United Kingdom to U.S.A.
Destination, Rates & Speeds

7.

Behringer, Richard/ Gertsenstein, Marina/ Nagy, Kristina/ Nagy, Andras
Published by Cold Spring Harbor Laboratory Pr (2013)
ISBN 10: 1936113007 ISBN 13: 9781936113002
New Hardcover Quantity Available: 1
Seller
Revaluation Books
(Exeter, United Kingdom)
Rating
[?]

Book Description Cold Spring Harbor Laboratory Pr, 2013. Hardcover. Book Condition: Brand New. 4th lab edition. 814 pages. 11.25x9.00x1.75 inches. In Stock. Bookseller Inventory # 1936113007

More Information About This Seller | Ask Bookseller a Question

Buy New
314.72
Convert Currency

Add to Basket

Shipping: 6
From United Kingdom to U.S.A.
Destination, Rates & Speeds

8.

Richard Behringer
Published by Cold Spring Harbor Laboratory Press (2013)
ISBN 10: 1936113007 ISBN 13: 9781936113002
Used Hardcover Quantity Available: 1
Seller
Books Express
(Portsmouth, NH, U.S.A.)
Rating
[?]

Book Description Cold Spring Harbor Laboratory Press, 2013. Hardcover. Book Condition: Good. 4 Lab. Ships with Tracking Number! INTERNATIONAL WORLDWIDE Shipping available. May not contain Access Codes or Supplements. Buy with confidence, excellent customer service!. Bookseller Inventory # 1936113007

More Information About This Seller | Ask Bookseller a Question

Buy Used
412.69
Convert Currency

Add to Basket

Shipping: FREE
Within U.S.A.
Destination, Rates & Speeds