This practical manual on protein research is designed to be a progression of experimental protocols that an inexperienced investigator may follow when embarking on a biochemical or biotechnology project. The reader is guided through the plethora of proven and productive methods for both soluble proteins and membrane or particulate-associated proteins. Special attention is given to providing optimalchoices from a range of methods available in a modern lab for a particular application. Over 180 protocols are included with materials lists, step-by-step instructions and comments on practical issues such as time considerations and safety issues. Each chapter includes troubleshooting tips, icons and references. Selected chapters and protocols may serve as lab courses in contmporary protein research techniques.
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New technologies and the manner in which scientific research is conducted have been fully incorporated into this thoroughly revised second edition of PROTEIN ANALYSIS AND PURIFICATION. Among the many topics introduced to this new edition are the use of robotics in the lab, the impact of the codification of the human genome, functional proteomics, mass spectrometry and the expanding use of bioinformatics as a tool to improve efficiency and productivity. The author has also included an annotated appendix of the educational materials and technical databases available online to enhance the researcher's benchside access to the latest resources. With solid explanations of the science asking the "why" and literally dozens of step-by-step protocols providing the "how", this new edition is a complete resource for the benchside.
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