Items related to Eukaryotic Cell Function and Growth: Regulation by...
Synopsis
Cell Fractionation by Centrifugation Methods.- - Introduction.- - Homogenization.- - Fractionation methods.- - Analysis of the fractions and interpretation of the results.- - Conclusion.- - References.- Binding of Ligands to Receptors: Theory.- - Introduction.- - Experimental approach to the hormone-receptor interaction.- - Molecular models of receptors.- - Graphical representations of saturation experiments.- - Methodologic errors and graphical representations.- - Conclusion.- - References.- On the Modes of Regulation of Intracellular Cyclic AMP: Desensitization of Adenylyl Cyclases to Hormonal Stimulation and Compartmentalization of Cyclic AMP.- - Introduction.- - Regulation of cyclic AMP levels by desensitization of adenylyl cyclase to hormonal stimulation.- - Regulation of cyclic AMP levels by compartmentalization of cyclic AMP.- - Nomenclature used.- - Abbreviations used.- - References.- The Interaction of Glucagon with Isolated Plasma Membranes of Rat Livers: The Relevancy of Measuring Binding Sites.- - Introduction.- - Transfer of hormonal message.- - Iodination of hormones.- - Measurement of binding.- - Relation between binding and adenylate cyclase.- - Effect of guanyl nucleotides on binding.- - How reliable is the measurement of binding as an indication of the first step in translation of the hormonal message ?.- - Degradation of glucagon by its target organ.- - The functional meaning of the receptor site.- - Summary.- - References.- Hormone Binding To Receptors: Vasopressin.- - Introduction.- - Methodological and technical considerations.- - Binding of lysine-vasopressin to pig kidney plasma membranes.- - Correlations between hormone binding and adenylate cyclase activation.- - References.- Effects of Choleragen and Fluoride on Adenylate Cyclase.- - Activation of adenylate cyclase by choleragen.- - Effects of fluoride on adenylate cyclase.- - References.- Stimulation of Adenylate Cyclase in the Thyroid.- - Introduction.- - TSH stimulation of adenylate cyclase in the thyroid.- - Immunoglobulin stimulation of adenylate cyclase in the thyroid.- - Prostaglandin stimulation of adenylate cyclase in the thyroid.- - Catecholamine stimulation of adenylate cyclase in the thyroid.- - Stimulation of adenylate cyclase in the thyroid by fluoride, bacterial products and biogenic amines.- - Conclusion.- - References.- Guanylate Cyclase.- - Introduction.- - Methods of assay.- - Distribution.- - General properties.- - Similarities in some properties of adenylate and guanylate cyclases.- - Effects of hormones and neurotransmitters on guanylate cyclase activity in cell free systems.- - References.- Regulation of Cyclic AMP Phosphodiesterase Activity.- - Effect of insulin on fat cell phosphodiesterase activity.- - Effects of intracellular cyclic AMP concentration on fat cell phosphodiesterase activity.- - Effects of prolonged elevation of cyclic AMP on phosphodiesterase activity.- - Effects of steroid hormones on phosphodiesterase activity.- - References.- Theoretical Simulation of the cAMP System.- - Introduction.- - Protein kinase and cyclic AMP.- - Consequences of the intracellular distribution of phosphodiesterases.- - Conclusion.- - References.- Control of Metabolic Processes by cAMP-Dependent Protein Phosphorylation.- - Introduction.- - Hormonal effects on cAMP-dependent protein kinases in intact tissues.- - Multiple forms of cAMP-dependent protein kinase.- - Effects of MgATP.- - Translocation.- - Phosphoprotein phosphatases.- - References.- Cyclic AMP and Cyclic GMP Binding in Tissues.- - Introduction.- - Characteristics of the cyclic AMP binding system.- - Cyclic GMP binding system.- - Hormonal regulation - intracellular titration of cyclic AMP bound to receptor proteins in rat diaphragm.- - Conclusions.- - References.- The Assay of Cyclic AMP in Biological Material.- - Introduction.- - Method of cyclic AMP analysis.- - Assay of cyclic AMP in biological samples.- - Summary.- - References.- Determination of Cyclic GMP in Biologic
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