Telomeres and Telomerase: Methods and Protocols: v. 191 (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 089603657X ISBN 13: 9780896036574

Publisher: Humana Press Inc., 2002

Synopsis

John A. Double and Michael J. Thompson have collected a critically important series of novel and essential techniques for studying telomeres and telomerase. These readily reproducible methods provide cutting-edge tools to identify, measure, and analyze telomeres, to determine telomerase expression at the RNA level, to determine telomerase activity, and to detect potential modifiers of this activity. The techniques for assaying telomerase activity range from standard radiological TRAP assays to nonradioactive methods, from non PCR-based methods to techniques using real-time PCR. "Telomeres and Telomerase: Methods and Protocols" provides the core array of productive techniques needed today to develop telomerase inhibitors or diagnostic/prognostic telomerase markers.

About this title

Review

"Among the many experimental protocols described in this book are methods for visualizing, manipulating, and assaying telomeres and telomerase activity. The protocols are written clearly and should serve as a useful laboratory resource for anyone wishing to learn how to study these entities. The book has a utilitarian look and feel, and while illustrations are not a major feature of the book, the ones that are included are of adequate quality. This book is intended and written as a laboratory guide manual. It would be quite useful for anyone doing bench research on telomeres and telomerase."-Doody's Health Sciences Book Review Journal

From the Back Cover

The discovery of telomerase expression in a majority of tumors and the recognition of its potential role in the aging process has dramatically raised its profile as a target for therapeutic intervention. In Telomeres and Telomerase: Methods and Protocols, John A. Double and Michael J. Thompson have collected a critically important series of novel and essential techniques for studying telomeres and telomerase. These readily reproducible protocols provide cutting-edge tools to identify, measure, and analyze telomeres, to determine telomerase expression at the RNA level, to determine telomerase activity, and to detect potential modifiers of this activity. The techniques for assaying telomerase activity range from standard radiological TRAP assays to nonradioactive methods, from non-PCR-based methods to techniques using real-time PCR. Written by investigators who have used the methods extensively, each protocol includes step-by-step instructions, tips on avoiding pitfalls, and invaluable notes that make all the difference to successful experimental outcomes. Broad-ranging and highly practical, Telomeres and Telomerase: Methods and Protocols provides molecular and cell biologists, cancer researchers, and cellular pathologists with the core array of productive techniques needed today to develop telomerase inhibitors and clinically valuable diagnostic/prognostic telomerase markers.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 2002
• Language: English
• ISBN 10: 089603657X
• ISBN 13: 9780896036574
• Binding: Hardcover
• Number of pages: 240
• Editor: Double John A., Thompson Michael