Differential Display Methods and Protocols: No. 85 (Methods in Molecular Biology) - Hardcover

Pardee

Hardcover

ISBN 10: 0896034895 ISBN 13: 9780896034891

Publisher: Humana Press Inc., 2009

Synopsis

Drs. Peng Liang and Arthur B. Pardee assemble for the first time a comprehensive review of the state of the art of their powerful new methodology and practical applications. The book's pioneering contributors describe all the major elements of this novel technology, including both RAP-PCR and DD using fluorescence detection, as well as their powerful strategy for identifying and cloning family-specific genes. They also provide numerous examples in which differentially expressed genes were successfully identified in diverse biological systems ranging from plants to songbirds to humans.

About this title

Review

"The variety of applications...is so broad that almost every newcomer...can use these protocols as a starting point for a specific study...highly recommended for purchase by everyone interested in the comparative gene expression, and especially to newcomers to the field." - Trends in Biotechnology

From the Back Cover

Drs. Peng Liang and Arthur B. Pardee have in Differential Display Methods and Protocols assembled for the first time a comprehensive review of the state of the art of their powerful new methodology and its practical applications. The book's pioneering contributors describe all the major elements of this novel technology, including both RAP-PCR and DD using fluorescence detection, as well as their powerful strategy for identifying and cloning family-specific genes. Also provided are numerous examples-along with detailed experimental procedures-in which differentially expressed genes have been successfully identified in diverse biological systems ranging from plants to songbirds to humans. Comprehensive and on the cutting-edge, Differential Display Methods and Protocols provides readers with precise new tools for studying how gene expression is exactly regulated throughout the development of a living organism, and how the failure of this intricate control mechanism leads to pathological complications. This novel and productive methodology, fully detailed here, is already playing a major role in the development of selective antagonists and inhibitors for treating cancer, cardiovascular disease, CNS disorders, and inflammation and tissue repair. Now scientists everywhere can successfully apply this powerful set of techniques using the step-by-step procedures of its innovators.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 2009
• ISBN 10: 0896034895
• ISBN 13: 9780896034891
• Binding: Hardcover
• Language: English
• Number of pages: 326