As in past editions, the basic philosophy of this extremely popular text is to present the principles and techniques of gene manipulation in sufficient detail to enable the non–specialist to understand them. However, in the three years since the previous edition, molecular biology has seen a dramatic increase in available techniques and applications. Every chapter in this new edition has been extensively revised or rewritten to take these developments into account – including a vastly expanded section on the polymerase chain reaction and its manifold applications, a new chapter examining the impact of recombinant DNA techniques on our understanding of human genetics, and extended coverage of gene transfer techniques.
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