Isothermal Titration Calorimetry in Enzymology: Techniques and Applications (Foundations and Frontiers in Enzymology) - Softcover

 
9780443218484: Isothermal Titration Calorimetry in Enzymology: Techniques and Applications (Foundations and Frontiers in Enzymology)
Softcover
ISBN 10: 044321848X ISBN 13: 9780443218484
Publisher: Academic Press, 2025

Synopsis

Isothermal Titration Calorimetry in Enzymology: Techniques and Applications provides a thorough, practical overview of ITC as a productive and powerful tool for quantifying enzyme catalysis and interactions with substrates, products, cofactors, and inhibitors. Here, global experts in the methodology discuss the full capabilities of ITC and how it may be applied in research, medicine, and industry and offer a basis to continue leveraging ITC as its uses expand and evolve. This book bridges the gap between research and application by giving extensive technical details on cutting edge ITC techniques across a wide swath of both ITC kinetics measurements and ITC thermodynamic measurements, alongside case studies illustrating the depth of knowledge that can be obtained. Applications discussed include measuring binding kinetics by ITC, analyzing ITC kinetic data, in vivo enzyme kinetics by ITC, analyzing enzyme kinetics in crowded solutions, studying catalysis of biological substrates, interpreting ITC binding curves, ITC in drug discovery, ITC in the development of cancer therapeutics, ITC analysis of aminoglycoside resistance enzymes, studying enzyme interactions with toxic metals, using ITC to study ubiquitination, and ITC studies of glycoside hydrolases, among others.

  • Describes and instructs in cutting-edge ITC experiments and analysis techniques
  • Covers both kinetics and thermodynamics-based ITC approaches
  • Features illustrative case studies across a wide range of applications
  • Includes chapter contributions from international experts in ITC

"synopsis" may belong to another edition of this title.

About the Authors

Professor Anthony Mittermaier works in the Department of Chemistry, McGill University, Montreal, Canada.

Justin Di Trani is an Assistant Professor at the University of Alberta, Department of Biochemistry, Edmonton, Canada

From the Back Cover

Isothermal Titration Calorimetry in Enzymology: Techniques and Applications provides a thorough, practical overview of ITC as a productive and powerful tool for quantifying enzyme catalysis and interactions with substrates, products, cofactors, and inhibitors. Here, global experts in the methodology discuss the full capabilities of ITC and how it may be applied in research, medicine, and industry and offer a basis to continue leveraging ITC as its uses expand and evolve. This book bridges the gap between research and application by giving extensive technical details on cutting edge ITC techniques across a wide swath of both ITC kinetics measurements and ITC thermodynamic measurements, alongside case studies illustrating the depth of knowledge that can be obtained. Applications discussed include measuring binding kinetics by ITC, analyzing ITC kinetic data, in vivo enzyme kinetics by ITC, analyzing enzyme kinetics in crowded solutions, studying catalysis of biological substrates, interpreting ITC binding curves, ITC in drug discovery, ITC in the development of cancer therapeutics, ITC analysis of aminoglycoside resistance enzymes, studying enzyme interactions with toxic metals, using ITC to study ubiquitination, and ITC studies of glycoside hydrolases, among others.

"About this title" may belong to another edition of this title.

Publisher
Academic Press
Publication date
2025
Language
English
ISBN 10 
044321848X
ISBN 13 
9780443218484
Binding
Paperback
Number of pages
354
Editor
Mittermaier