Immunoassays: A Practical Approach: No.228 - Hardcover

Gosling, J.R.G.

Hardcover

ISBN 10: 0199637113 ISBN 13: 9780199637119

Publisher: Oxford University Press, 2000

Synopsis

This practical volume is designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are also described. The text goes on to explain solid-phase reagents, standards and immunogens, with procedures for their preparation. Most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.

About this title

Review

"This book is very useful not only for immunologists but also for amny specialists in chemistry and biology and is certainly recommendable"Immunological Investigations

Edition details

• Publisher: Oxford University Press
• Publication date: 2000
• ISBN 10: 0199637113
• ISBN 13: 9780199637119
• Binding: Hardcover
• Language: English
• Number of pages: 324