Directed Mutagenesis: A Practical Approach - Hardcover

McPherson, M. J.

Hardcover

ISBN 10: 0199631417 ISBN 13: 9780199631414

Publisher: Oxford University Press, 1991

Synopsis

Directed mutagenesis has emerged as an essential tool in both genetic and protein engineering by facilitating both molecular analysis and the modification of the structural and functional properties of cloned DNA sequences. Techniques have been refined to the point where virtually any desired change or series of changes in the nucleotide sequence may be rapidly introduced. Several methods also exist for the introduction of random rather than targeted mutations, thus allowing the identification of structurally or functionally important regions that might not otherwise be detected. This book draws together a wide range of methods for the efficient directed mutagenesis of cloned DNA sequences. It provides up-to-date protocols and practical advice that should be of use to the beginner who wishes to be guided through the available approaches, and to the experienced researcher interested in exploring alternative procedures.

About this title

Review

'The text is presented in such a way as to be suitable for both beginner and expert, especially those interested in exploring alternative procedures.' ASLIB Booklist, Vol.56, No.10, October 1991

'This book draws together a wide range of methods for the efficient directed mutagenesis of cloned DNA sequences. It provides up-to-date protocols and practical advice that will prove valuable to the beginner who wishes to be guided through the available approaches, and to the experienced researcher interested in exploring alternative procedures.'Biochimica Clinica, 1992, Vol. 16, No. 6

'The latest in the Practical Approach series ... is all that you would expect from its long line of predecessors. An illustrious list of contributors includes many leaders in their respective fields as well as the pioneers of the techniques they describe. This alone lends authority to and instils confidence in each method.'Daniel C. Emery, University of Bristol, BioEssays, Vol. 15, No. 4 - April 1993

Edition details

• Publisher: Oxford University Press
• Publication date: 1991
• ISBN 10: 0199631417
• ISBN 13: 9780199631414
• Binding: Hardcover
• Language: English
• Number of pages: 277