Unraveling DNA: Molecular Biology for the Laboratory

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9780132700344: Unraveling DNA: Molecular Biology for the Laboratory

This manual encompasses an integrated series of molecular biology laboratory exercises that involve the cloning and analysis of the bioluminescence (lux) genes from the marine bacterium Vibrio fischeri. The manual is divided into discrete units with each demonstrating one or more aspects of the cloning project. The manual is based on one of nature's most fascinating biological phenomenon: the biological production of light. This results in a recurrent theme of interest and makes the project very relevant to interdisciplinary topics such as fish symbiosis, biochemistry, biophysics, etc. Includes instruction in the basic techniques of modern molecular biology: DNA isolation and analysis, DNA restriction, agarose gel electrophoresis, ligations, transformation of recombinant DNA, preparation and screening a genomic library, restriction mapping, Southern blotting, hybridization, DNA sequencing, pulsed field gel electrophoresis. Designed for a one semester course in Molecular Biology. Also appropriate for a molecular biology component of Microbial Genetics, Genetics, Biochemistry, or Advanced Microbiology courses.

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This innovative manual introduces students to all of the basic techniques of modern molecular biology using an integrated series of laboratory exercises that involve the cloning and analysis of the bioluminescence (lux) genes from the marine bacterium Vibrio fischeri. By organizing the exercises as part of a major cloning project, students get the sense of performing a complete cloning project, rather than just learning a collection of procedures. The manual is divided into discrete units with each demonstrating one or more aspects of the cloning project. Collectively, the entire series of exercises requires approximately three quarters to one full semester to complete with two laboratory periods per week. Smaller portions of the manuals are easily adapted to fewer lab periods.

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Winfrey, Michael R., Rott, Marc A., Wort
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Book Description Pearson Education (US), United States, 1997. Paperback. Book Condition: New. New.. Language: English . This book usually ship within 10-15 business days and we will endeavor to dispatch orders quicker than this where possible. Brand New Book. Appropriate for a variety of undergraduate courses covering biology and genetics, including Molecular Biology, Microbial Genetics, Genetics (portion on Molecular Genetics), Biochemistry (portion on Molecular Genetics), Advanced Bacteriology or Microbiology. This innovative manual introduces students to all of the basic techniques of modern molecular biology using an integrated series of laboratory exercises that involve the cloning and analysis of the bioluminescence (lux) genes from the marine bacterium Vibrio fischeri. By organizing the exercises as part of a major cloning project, students get the sense of performing a complete cloning project, rather than just learning a collection of procedures. The manual is divided into discrete units with each demonstrating one or more aspects of the cloning project. Collectively, the entire series of exercises requires approximately three quarters to one full semester to complete with two laboratory periods per week. Smaller portions of the manuals are easily adapted to fewer lab periods. Bookseller Inventory # BTE9780132700344

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Book Description 1997. Paperback. Book Condition: New. 0005th. Paperback. Using an integrated series of laboratory exercises that involve the cloning and analysis of the bioluminescence (lux) genes from the marine bacterium Vibrio fischeri, this man.Shipping may be from our Sydney, NSW warehouse or from our UK or US warehouse, depending on stock availability. 400 pages. 0.894. Bookseller Inventory # 9780132700344

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