Techniques for Microbiology: A Student Handbook

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9780132240116: Techniques for Microbiology: A Student Handbook

Lammert’s approach is visual and incorporates “voice balloons” that keep the student focused on the process described. The techniques are those that will be used frequently for studying microbes in the laboratory, and include those identified by the American Society for Microbiology in its recommendations for the Microbiology Laboratory Core Curriculum (recommendations in which the author participated).

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Book Description Benjamin Cummings, 2006. Book Condition: New. Brand New, Unread Copy in Perfect Condition. A+ Customer Service! Summary: Unit I Safety first! Safety in the microbiology laboratory Universal precautions Unit II Culturing bacteria and aseptic techniques Preparing culture media Sterilization The autoclave Dry heat Aseptic transfer of bacteria Adjusting the gas burner Preparing bacterial cultures in broth tubes and on agar slants Transfer colony from plate to broth and to agar slant Isolation of bacteria Preparing a pour plate Preparing a streak plate Preparing a spread plate Characteristic features of bacterial growth in broth and on agar Maintenance and storage of stock cultures Culturing anaerobic bacteria Unit III Visualizing bacteria Effective use and responsible care of the light microscope Measuring microscopic cells Preparing a hanging-drop slide Preparing a bacterial smear Preparing a simple stain Preparing a Gram stain Preparing an acid-fast stain Preparing a negative stain Preparing a capsule stain Preparing an endospore stain Unit IV Enzyme-based tests for the identification of bacteria Hydrolytic (digestive) enzymes Starch hydrolysis Casein hydrolysis Gelatin hydrolysis Lipid hydrolysis (tributyrin and spirit blue agar) DNA hydrolysis Utilization of carbohydrates Fermentation of carbohydrates (Durham tube) Methyl red test (mixed fermentation) Voges-Proskauer test (butanediol fermentation) Citrate utilization Oxidation-fermentation (O-F) glucose test Degradation of amino acids Indole test H2shy;S production Phenylalanine deamination Decarboxylase test Respiration tests Catalase test Oxidase test Nitrate reduction Miscellaneous tests Triple sugar iron (TSI) test Urea hydrolysis Litmus milk reactions Motility assay Coagulase production Selective and/or differential media Blood agar Eosin methylene blue (EMB) agar Mannitol-salt agar MacConkey agar Phenylethyl alcohol (PEA) agar Unit V Counting microbes Direct microscopic counting with the Petroff-Hausser chamber Preparing a standard plate count of bacteria Using Turbidemetry to estimate cell density Plaque assay for determining bacteriophage titer Unit VI Measuring effectiveness of antibacterial chemicals Kirby-Bauer method for sensitivity of bacteria to antibacterial medicines Evaluating antibacterial chemicals: The disk-diffusion method. Bookseller Inventory # ABE_book_new_0132240114

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