Transgenic Animal Technology: A Laboratory Handbook

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9780124104907: Transgenic Animal Technology: A Laboratory Handbook

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. * Contains a comprehensive collection of transgenic animal and gene transfer methods* Discusses background and introduction to techniques and animal systems* Teaches practical step-by-step protocols* Fully revised with updates to reflect state-of-the-art technology and associated changes to date

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Review:

The handbook will be a valuable resource for two audiences, one seeking a broad appreciation for transgenic animal technology, which can be gained by reading the handbook cover to cover, and the other seeking concise descriptions of focused techniques, which can be found by selection of specific chapters. --Transgenics (2003)

This handbook would be valuable to the investigators producing transgenic animal models. It is well organized and the handbook provides a thorough description of current animal transgenic technology across a host of animals. --Analytical Biochemistry (2003)

About the Author:

Dr. Pinkert is an Associate Professor in the Department of Comparative Medicine and Director of the Transgenic Animal/ES Cell Resource at the University of Alabama at Birmingham. He earned his B.S. from Colorado State University, M.S. from Southern Illinois, and Ph.D. in 1983 from the University of Georgia, followed by postdoctoral training at the University of Pennsylvania. He was an Assistant Professor at the University of Missouri and then worked at DNX, Inc. (where his proposal for the National Transgenic Development Facility for the National Institute of Child Health and Human Development was awarded and implemented) before joining the UAB faculty. Dr. Pinkert's primary research efforts involve gene transfer, expression, and regulation using transgenic animal modeling. His work has illustrated the potential of modifying the immune system, enhancing growth performance, and the feasibility of molecular farming. He has published extensively on transgenic modeling, reproductive biology, and embryology of laboratory and domestic animals. He serves as an Editro of Transgenic Research and has served as an ad hoc reviewer or consultant for several journals, government agencies, and companies.

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