Adult Stem Cells (Methods in Enzymology): Volume 419 - Hardcover

Hardcover

ISBN 10: 0123736501 ISBN 13: 9780123736505

Publisher: Academic Press, 2006

Synopsis

This is the second of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques focusing on adult stem cells, and written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories.

• Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering
• Presents the latest most innovative technologies
• Addresses therapeutic relevance including FDA compliance and tissue engineering

About this title

Review:

A collection of methods and techniques for working with stem cells.

Synopsis:

This is the second of three planned volumes in the "Methods in Enzymology" series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other.

Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories. It provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering. It presents the latest most innovative technologies; and addresses therapeutic relevance including FDA compliance and tissue engineering.

About this edition

• Publisher: Academic Press
• Publication date: 2006
• ISBN 10: 0123736501
• ISBN 13: 9780123736505
• Binding: Hardcover
• Number of pages: 576
• Editor: Klimanskaya Irina, Lanza Robert