Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas: Molecular Genetics; Lung and Breast Carcinomas: Molecular Genetics - Lung and Breast Carcinomas: 1 - Hardcover

Hardcover

ISBN 10: 0123339413 ISBN 13: 9780123339416

Publisher: Academic Press, 2004

Synopsis

The various cell types have traditionally been recognized and classified according to their appearance in the light microscope following the process of fixing, processing, sectioning, and staining tissues that is known as histology. Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system.

Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today, ensuring a wide audience for this book.

• The only book available that translates molecular genetics into cancer diagnosis
• Provides the readers with tools necessary to perform and optimize sensitive, powerful techniques, including immunohistochemistry and fluorescence in situ hybridization, used in tumor diagnosis
• Written by experts in this field, the book provides theoretical considerations as well as practical approaches to carry out effectively these techniques
• Offers suggestions, tips, cautions, and guidelines to avoid artifacts and misdiagnosis
• Introduces new techniques to detect genes and proteins involved in the initiation and progression of cancer
• Covers the latest developments and a wide range of applications to the detection of antigens and single-copy DNA and RNA
• Written in a uniform format, each chapter includes Introduction, Materials required, step-by-step detailed Methods, Results, Discussion, and comprehensive up-to-date References

About this title

Review:

"...this handbook is of unparalleled value as a reference book and should be on the shelf of all serious investigators in the field... Its value lies in having an immediate reference source at hand, replete with bibliography, for a particular research project, or occasionally for a particularly difficult clinical issue. In these areas, there are simply no competitors in print..." --Clive R. Taylor, Dept of Pathology, The Keck School of Medicine at the University of Southern California, Los Angeles, in APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY (June 2006)

"...this book is a valuable reference for medical students, medical technician, pathologists, and clinician alike who are interested in tumor biology or translational research." --HUMAN PATHOLOGY (July 2005)

"Volume 1 of this three-volume Elsevier handbook, edited by M.A. Hayat on the 'Immunohistochemistry and in situ Hybridization of Human Carcinomas' is impressive. ...The technical detail included in many contributions surpasses that available from the scientific and clinical journals; all provide a depth of up-to-date knowledge based upon the authors' experience and expertise. Every large hospital and medical school library should be persuaded to buy a copy of this book." --MICRON (2005)

"...Professor Hayat’s handbook, Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas has the great potential of becoming the handbook of choice for clinician and basic scientists including medical and biological students and biomedical technologists." --Akhouri A. Sinha, Ph.D., Professor of Genetics, Cell Biology and Development, University of Minnesota and Research Scientist at the VA Medical Center Minneapolis, MN

From the Back Cover:

Classical histology has been augmented by
immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system.

This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today. This book will be of particular interest to pathologists and molecular pathologists conducting both academic and/or clinical research.

*It is the only book that translates molecular genetics into cancer diagnosis
*The contributors are internationally-recognized authorities in their field of specialty
*The results of each immunohistochemical and in situ hybridization method are presented in the form of color illustrations
*Methods discussed were either developed or refined by the contributors in their laboratories
*Methods are presented in step-by-step manner, and the reader does not require outside help

About this edition

• Publisher: Academic Press
• Publication date: 2004
• ISBN 10: 0123339413
• ISBN 13: 9780123339416
• Binding: Hardcover
• Number of pages: 556
• Editor: Hayat M. A.