This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
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Praise for the Series "The Methods in Enzymology series represents the gold-standard." (Neuroscience)
"Incomparably useful." (Analytical Biochemistry)
"...including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." (Bio/Technology)
"...It should be on the shelves of all libraries in the world as a whole collection." (Chemistry in Industry)
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation..." (American Society of Microbiology News)
"If we had some way to find the work most often consulted in the laboratory, it could well be...Methods in Enzymology." (Enzymologia)
"A series that has established itself as a definitive reference for biochemists." (Journal of Chromatography)From the Back Cover:
This book covers the significant new developments that have expanded the scope of enzyme chemistry. At the most fundamental level, Nature's use of nucleic acids as enzymes became evident as did the ability of enzyme chemists to design catalytic antibodies as synthetic enzymes.
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