Hormone Action: Neuroendocrine Peptides Pt.H (Methods in Enzymology)

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9780121820039: Hormone Action: Neuroendocrine Peptides Pt.H (Methods in Enzymology)

From the Preface: A survey of the current biological literature will convince even the most skeptical individual of the substantive and pervasive advances that have occurred in neuroendocrinology. It was the observation that this rapid growth resulted in large part from methodological improvements which first suggested the need for this volume. Some of the advances can be easily recognized. High pressure liquid chromatography, once a tool of the solvent chemist, is now a routine analytical and preparative method in biological laboratories. From the Preface: It has enhanced the speed and resolution with which neuroendocrine substances can be separated. Likewise, peptide synthesis, modification, and analysis are no longer the ken of those whose work is dedicated solely to this task, rather, as one of the authors' put it, 'almost anyone can synthesize a peptide.' Analytical advances have extended the sensitivity and specificity of assay techniques. Culture techniques have been devised which allow us to purify and maintain isolated cells and tissue. In turn, these enhancements have revealed the presence and biological roles of new neuroendocrine substances; in other cases, novel and surprising sites of action have been identified for substances already described.

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Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE "Incomparably useful." --ANALYTICAL BIOCHEMISTRY "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS "If we had some way to find the work most often consulted in the laboratory, it could well be Colowick and Kaplan's multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA "A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

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