Quantitative Imaging in Cell Biology: Methods in Cell Biology: 123: Volume 123 - Hardcover

Hardcover

ISBN 10: 0124201385 ISBN 13: 9780124201385

Publisher: Academic Press, 2014

Synopsis

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. 

The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.

• Covers sections on model systems and functional studies, imaging-based approaches and emerging studies
• Chapters are written by experts in the field
• Cutting-edge material

About this title

Review:

This new volume of Methods in Cell Biology looks at methods for quantitative imaging in cell biology.

About this edition

• Publisher: Academic Press
• Publication date: 2014
• ISBN 10: 0124201385
• ISBN 13: 9780124201385
• Binding: Hardcover
• Number of pages: 588
• Editor: Waters Jennifer, Wittmann Torsten