Membrane Protein Purification and Crystallization: A Practical Guide - Softcover

9780123617767: Membrane Protein Purification and Crystallization: A Practical Guide

Softcover

ISBN 10: 0123617766 ISBN 13: 9780123617767

Publisher: Academic Press, 2003

Intended for bench scientists working in the fields of biochemistry, biology, and proteomic research, this guide presents isolation and crystallization techniques, emphasizing the critical aspects unique to membrane proteins. It also explains the principles of the methods and provides protocols of general use.

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From the Back Cover:

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first manual compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.

Key Features
* Provides guidelines for isolation and crystallization of membrane proteins
* Presents a comprehensive guide to 2D-crystallization of membrane proteins
* Describes production and use of antibody fragments
* Emphasizes recent progress in purification of recombinant membrane proteins
* Gives detailed protocols

New Contributions to the Second Edition
* An entire section devoted to crystallization of membrane proteins
* Summarizes recent developments of Blue-Native PAGE
* Production and purification of recombinant membrane proteins
* Preparative Isoelectric Focussing
* Purification of affinity-epitope tagged G-protein coupled receptors
* Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D crystallization
* Purification of the yeast cytochrome bc1 complex

The Second Edition contains 75% new content!|This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first manual compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.

Key Features
* Provides guidelines for isolation and crystallization of membrane proteins
* Presents a comprehensive guide to 2D-crystallization of membrane proteins
* Describes production and use of antibody fragments
* Emphasizes recent progress in purification of recombinant membrane proteins
* Gives detailed protocols

New Contributions to the Second Edition
* An entire section devoted to crystallization of membrane proteins
* Summarizes recent developments of Blue-Native PAGE
* Production and purification of recombinant membrane proteins
* Preparative Isoelectric Focussing
* Purification of affinity-epitope tagged G-protein coupled receptors
* Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D crystallization
* Purification of the yeast cytochrome bc1 complex

The Second Edition contains 75% new content!

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